| extract promotes M2 polarization and reduces oligomeric amyloid-β-induced inflammatory reactions in microglial cells.|
Sun ZQ, Liu JF, Luo W, Wong CH, So KF, Hu Y, Chiu K.
Neural regeneration research. 2022; 17(1): 203-209
Lycium barbarum (LB) is a traditional Chinese medicine that has been demonstrated to exhibit a wide variety of biological functions, such as antioxidation, neuroprotection, and immune modulation. One of the main mechanisms of Alzheimer's disease is that microglia activated by amyloid beta (Abeta) transform from the resting state to an M1 state and release pro-inflammatory cytokines to the surrounding environment. In the present study, immortalized microglial cells were pretreated with L. barbarum extract for 1 hour and then treated with oligomeric Abeta for 23 hours. The results showed that LB extract significantly increased the survival of oligomeric Abeta-induced microglial cells, downregulated the expression of M1 pro-inflammatory markers (inducible nitric oxide synthase, tumor necrosis factor alpha, interleukin-6, and interleukin-1beta), and upregulated the expression of M2 anti-inflammatory markers (arginase-1, chitinase-like protein 3, and interleukin-4). LB extract also inhibited the oligomeric Abeta-induced secretion of tumor necrosis factor alpha, interleukin-6, and interleukin-1beta in microglial cells. The results of in vitro cytological experiments suggest that, in microglial cells, LB extract can inhibit oligomeric Abeta-induced M1 polarization and concomitant inflammatory reactions, and promote M2 polarization.