Melatonin sensitizes esophageal cancer cells to 5‑fluorouracil via promotion of apoptosis by regulating EZH2 expression.
Zhang M, Zhang M, Li R, Zhang R, Zhang Y.
Oncology reports. 2021; 45(4): 1

Abstract

The present study aimed to investigate the effects of melatonin (MLT) and 5fluorouracil (5FU) combination on the chemotherapeutic effect of 5FU in esophageal cancer, and determine the potential molecular mechanisms. The effects of MLT and 5FU combination on cell proliferation, cell migration and invasion, and cell apoptosis were detected by Cell Counting Kit8, Transwell assays and flow cytometric analysis, respectively. Quantitative PCR and western blotting were performed for mRNA and protein quantification, respectively. The present study revealed that MLT significantly inhibited cell activity in a dosedependent manner and MLT significantly enhanced 5FUmediated inhibition of cell proliferation in esophageal cancer cells. Compared with the 5FU group, the MLT and 5FU combination group significantly inhibited the invasion and migration of EC9706 and EC109 cells. The present study also revealed that MLT and 5FU synergistically promoted apoptosis via activation of the caspasedependent apoptosis pathway. Histone-lysine Nmethyltransferase EZH2 (EZH2) was highly expressed in esophageal cancer tissues and cells and its high expression promoted esophageal cancer progression. MLT and 5FU combination inhibited cell proliferation and promoted apoptosis by regulating EZH2 expression. In conclusion, MLT enhanced 5FUmediated inhibition of cell proliferation via promotion of apoptosis by regulating EZH2 expression in esophageal cancer.



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